Wanda Janaeska: A morphology-based classification algorithm for identifying caveolae...

NMIN HQP Wanda Janaeska (University of Toronto) presents on her research in Dr. Warren Chan’s lab: A morphology-based classification algorithm for identifying caveolae, clathrin vesicles, and macropinosomes on electron microscopy images.


Transmission electron microscopy has the resolution necessary to resolve nanoparticle-containing vesicles in cells. However, it is challenging to manually differentiate these vesicles as caveolae, clathrin, or macropinosomes. Here, we develop a classification algorithm that classifies endocytic vesicles imaged with TEM. Characterizing nanoparticle-containing vesicles helps us understand which endocytosis pathways are used by cells to transport nanoparticles.