DETAILS - Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. (In-vitro Swedish Study)

DETAILS - Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. (In-vitro Swedish Study)

An accepted and published Swedish study demonstrates for the first time the presence of Pfizer-BionTech vaccine DNA in the cell. It is reverse transcribed by LINE-1 proteins of the cells. They are not sure if it gets integrated in our DNA or not. Let's review the details of their method.

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Chapters
00:00 introduction
00:45 Link to the summary
01:00 Review of the reference material
02:19 Cell preparation for the vaccine
03:35 Vaccine addition to the cells
06:00 RT-PCR for find RNA and then DNA
13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA
18:27 Measuring the volume of proteins in the cytosol and the nucleus
22:35 DNA purification and verification
28:41 Results
28:42 LNP enters the liver cells efficiently
29:33 LINE-1 gene is expressed
30:46 ORF-1 found in cytosol and the nucleus
36:01 DNA from the vaccine found in the cell
43:30 Questions not answered



Disclaimer:
This video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. The content provided in this video is for informational and educational purposes only.
Please consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. No information in this video should ever be considered as a substitute for advice from a healthcare professional.

URL list from Friday, Feb. 25 2022

CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML
https://www.mdpi.com/1467-3045/44/3/73/htm

Frontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics
https://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes.

URL list from Monday, Feb. 28 2022

CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML
https://www.mdpi.com/1467-3045/44/3/73/htm

LINE-1 protein localization and functional dynamics during the cell cycle | eLife
https://elifesciences.org/articles/30058

Time evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv
https://www.biorxiv.org/content/10.1101/2021.08.20.457104v1

Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues - PubMed
https://pubmed.ncbi.nlm.nih.gov/33958444/

No evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues - PMC
https://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC8379926/

Overview of Reverse Transcription - Retroviruses - NCBI Bookshelf
https://www.ncbi.nlm.nih.gov/books/NBK19424/

Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology
https://www.nature.com/articles/s41564-020-0735-8

Chimeric RNA - Wikipedia
https://en.wikipedia.org/wiki/Chimeric_RNA

Benchtop Automation for Immunohistochemistry (IHC), ISH & Special Stains (SS) - NanoMtrx 300 - YouTube
https://www.youtube.com/watch?v=3IRl0kbR9-o&ab_channel=BioGenexLaboratories

Schrödinger's cat - Wikipedia
https://en.wikipedia.org/wiki/Schr%C3%B6dinger%27s_cat